“STUDY OF CITOTOXIC PARAMETERS OF Verbascum thapsus EXTRACS ON VERO CELLS”.

ESCOBAR FRANCO MATÍAS; GALLOTTI VALERIA; SABINI MARÍA CAROLA; TORRES CRISTINA VANESA; TONN CARLOS EUGENIO; GIORDANO OSCAR; SABINI LILIANA INÉS

TAFÍ DEL VALLE, TUCUMÁN. ARGENTINA

Congreso; BIOCELL; 2009

ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN

STUDY OF CITOTOXIC PARAMETERS OF Verbascum thapsus EXTRACS ON VERO CELLS. Escobar1 F., Gallotti1 V., Sabini2 C., Torres1 C., Tonn3 C.,Giordano3 O., Sabini1, L. 1:FCEFQyN, UNRC. 2:UNC. 3:UNSL. lsabini@exa.unrc.edu.ar Introduction: Verbascum thapsus L. Scrophulariacea (verbascum) is a medicinal plant native from Europe and Asia. It has been described expectorant, astringent, febrifugue, descongestive and antialergic properties. However, the use of this plant in vivo needs the evaluation of citotoxic parameters to guarantee it safety. Aims: To study the citotoxicity of 5 vegetable extracts (VE) from V.thapsus. Materials and Methods: n-hexanic (HE), cloroformic (CE), methanolic (ME), cold aqueous (CAE) and hot aqueous extracts (HAE) were obtained from leaves. They were diluted in MEM-Eagle-Earle + 2% BFS and seeded on Vero cells monolayers. MCNC and CC50 by neutro red and MTT were calculated. Results: It was observed a correlation between the 3 methods employed, so a toxicity order of the VE could be defined: EAF <EAC <EH <EM <EC. Conclusions: The study of MCNC by the neutral red uptake (NRU) and MTT salt reduction methods was useful to define toxic concentration ranges. Both methods were very sensitive for the determination of extracts toxicity. CC50 values obtained with NRU assay were lower than MTT salt reduction. These results suggest that the citotoxic action of the VE mainly happened at a lisosomal level and not a mitocondrial one. The knowledge of the toxic ability of V.thapsus will help to define the selectivity action of future therapeutic formulations of this VE on eucariotic cells.