INVESTIGADORES
SABINI Maria Carola
artículos
Título:
Preventive effects of the antioxidant and antigenotoxic Achyrocline satureioides extract against zearalenone-induced mammal cytogenotoxicity and histological damage
Autor/es:
SABINI MARÍA CAROLA; CARIDDI LAURA NOELIA; ESCOBAR FRANCO MATÍAS; MAÑAS FERNANDO; ROMA DARDO; MENIS CANDELA FLORENCIA; BAGNIS GUILLERMO; SORIA ELIO ANDRÉS; SABINI LILIANA INÉS; DALCERO ANA MARIA
Revista:
World Mycotoxin Journal
Editorial:
Wageningen Academic Publishers
Referencias:
Año: 2021 vol. 14 p. 401 - 409
ISSN:
1875-0710
Resumen:
Zearalenone (ZEN), a Fusarium´s mycotoxin, is immunotoxic, genotoxic, hepatonephrotoxic and, affects the reproductive system. ZEN induces toxic and genotoxic effects on humans and other animals. Achyrocline satureioides has several medicinal properties. Moreover, the aqueous extract of A. satureioides is a safe agent that exerts low cytotoxicity and no genotoxicity. This extract is a promissory candidate to counteract ZEN effects. The present study aimed to investigate the capacity of cold aqueous extract from A. satureioides to protect against ZEN multi-target toxicity in different experimental mammal models. Anticytotoxicity was evaluated by neutral red uptake and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium reduction assays. Comet assay and micronuclei test, oxidative stress (TBARs), and histopathological damage were evaluated in Balb/C mice. Anticytotoxic studies indicated that cold aqueous extract (100 and 300 µg/mL) protected from damage induced by ZEN (50 µg/mL) on Vero cells. In vivo studies indicated that ZEN (40 mg/kg b.w.) induced an increase of genotoxicity: micronuclei (34 MNPCE/1000PCE) and increase of damage (tail moment) in blood cells. Also, it increased lipid peroxidation in liver and kidneys and generated several histopathological alterations in both organs. Cold aqueous extract (100 mg/kg b.w.) protected from genotoxicity induced by ZEN in both tests. Cold aqueous extract, also, reduced the lipid peroxidation and histopathological damage in liver and kidneys. In conclusion, the cold aqueous extract of Achyrocline satureioides that contains bioactive flavonoids prevents the multi-target toxicity induced by zearalenone improving all the parameters evaluated in vitro and in vivo, which is a valuable and original finding in order to develop future treatments for human and veterinary medicine.