Array-Based Comparative Study of Apomictic and Sexual Eragrostis Curvula Genotypes

SELVA JP; GARBUS I; PASTEN C; CARBALLO J; ZAPPACOSTA D; PELLINO M; SHARBEL T; ECHENIQUE V

San Diego

Conferencia; Plant and Animal Conference; 2018

PAG

Apomixis is an asexual reproductive process that results in seeds containing maternal clones since meiotic reduction and egg cell fertilization does not occur. Apomixis is common in polyploid grasses, and poorly represented in crop species of economic interest. A key goal in understanding the molecular basis of apomixis is the possible transference of this trait to species of agronomic relevance. Weeping lovegrass (Eragrostis curvula [Schrad.] Nees) is a perennial grass native to Southern Africa that reproduces by diplosporous apomixis. A custom 60-mer spots Agilent array was designed with 970 k probes based on a reference transcriptome constructed from inflorescence of sexual and apomictic genotypes.