INVESTIGADORES
ECHENIQUE Carmen Viviana
congresos y reuniones científicas
Título:
Evaluation of leaf rust resistance based on the Lr14a gene (7BL QTL) in a collection of durum wheat materials in different environments from Argentina.
Autor/es:
RONCALLO P; CAMPOS P; DREISIGACKER S; LARSEN AO; MATUS I; MAICO N; MONTICELLI N; ROCHÓN M; CUESTAS JM; GONZALEZ L; JENSEN C; MIRANDA R; ECHENIQUE V
Lugar:
Foz de Iguazú
Reunión:
Congreso; XI International Plant Molecular Biology Congress; 2015
Institución organizadora:
IPMB
Resumen:
Leaf rust (Puccinia triticina Rob. ex. Desm.) causes the highest yield losses in durum wheat worldwide. One of the sources of resistance is located on 7BL conferred by Lr14a gene. The objectives of this work were i) evaluate under field conditions with and without inoculation and by inoculation in seedling stage at greenhouse the variability for this trait in a collection of 168 durum wheat materials, ii) assess the effect of the haplotype for the 7BL region on the durable resistance to leaf rust. Three field trials sown in 2014 (Pieres, Barrow and Cabildo) were evaluated for leaf rust response without inoculation and an additional one (Bordenave) was conducted with inoculation using virulent leaf rust isolates. The SSR markers Xgwm146 and Xgwm344 and the KASP marker ubw14 were analyzed in the collection and 11 materials showed a common haplotype corresponding with the marker profiles of Llareta, Creso, Italo and Sachem, showing complete resistance under field conditions. Sachem presented one additional band (low-weight) at Xgwm344. The Argentinean varieties Bonaerense INTA Carilo and Bonaerense INTA Quillen shared these alleles but showed not to be resistant after inoculation. Fifty two materials exhibited complete resistance or traces of infection under natural field conditions and 35 maintained this condition after inoculation with a mixture of isolates collected in Argentina. These results validate the use of the Lr14a gene as a leaf rust resistance source and showed the existence of different genes in the durum wheat collection conferring good level of resistance after artificial infection