INVESTIGADORES
ECHENIQUE Carmen Viviana
congresos y reuniones científicas
Título:
De novo transcriptome sequencing and assembly for studying apomixis related expression in Eragrostis curvula
Autor/es:
GARBUS I; ROMERO J; SELVA JP; VIVIANA ECHENIQUE
Lugar:
Foz de IguazĂș
Reunión:
Congreso; XI International Plant Molecular Biology Congress; 2015
Institución organizadora:
IPMB
Resumen:
Eragrostis curvula represents an important cultivated forage resource in semiarid regions which reproduce by pseudogamous diplosporous apomixis. We previously produced an EST collection from panicles of a series of genetically related E. curvula lines. In this work, next generation sequencing technologies were used to increase the transcriptome sequences available aiming to generate an useful resource for gene annotation and discovery of this species. Thus, four inflorescence cDNA libraries, two from the apomictic cultivar Tanganyika and two from the sexual cultivar OTA, were generated though Roche 454 pyrosequencing, leading to 2,617,197 raw reads with an average length ~364 bp. Due to the lack of E.