INVESTIGADORES
DINOLFO Maria Ines
artículos
Título:
Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
Autor/es:
ORTEGA L.M.; DINOLFO M.I.; ASTORECA A.L.; ALBERIONE E.J.; STENGLEIN S.A.; ALCONADA T.M.
Revista:
MYCOLOGICAL PROGRESS
Editorial:
SPRINGER HEIDELBERG
Referencias:
Lugar: HEIDELBERG; Año: 2016 vol. 15 p. 1 - 8
ISSN:
1617-416X
Resumen:
Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto isolates obtained from wheat samples of 2009, 2010 and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using Inter-Simple Sequence Repeats (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. The 95% of the 68 F. graminearum sensu stricto isolates obtained in this study showed a different banding pattern with ISSR molecular markers and a high variability was detected within population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741µg/g. The results suggest that F. graminearum s.s. population vary significantly in both genetic and toxin production at a limited sampled area.