INVESTIGADORES
DEGANO Alicia Laura
congresos y reuniones científicas
Título:
PRESINAPTIC DYSFUNCTION IN DIFFERENT BRAIN AREASOF RATS WITH EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE).
Autor/es:
CHANADAY NL; VILCAES AA; DEGANO AL; ROTH GA
Lugar:
Huerta Grande, Córdoba. Argentina
Reunión:
Congreso; XXVII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
EAEis a model that mimics many of the clinical and pathological features of multiple sclerosis (MS). While MS and EAE research has been mostly focused on spinal cord inflammation/demyelination, the extent and implications of gray matterdamage are still not fully understood. Herein we show that Ca2+-dependent glutamate release from isolated synaptosomes is specifically impaired in frontal cortex and striatum from rats with EAE, with no changes in total glutamate content. Synapsin I is necessary for synaptic vesicle mobilization to the active zone as well as for vesicle recycling and anchoring to the actin cytoskeleton. The first event is regulated by CaMKII phosphorylation of P-sites 1 to 3 and the latter is triggered by Erk1/2 (MAPK) phosphorylation of P-sites 4 and 5. In synaptosomes from EAE rats, depolarization-induced phosphorylation on P-site 3 of Synapsin I is reduced whereas Erk1/2 autophosphorylation is increased. All these changes rapidly reverse when the animals begin to recover from the clinical signs of the disease. These data indicate that presynaptic machinery is affected in EAE, unraveling in part the mechanism of neural dysfunction. Since this occurs in brain areas related to motor control and learning it might contribute to the disease progress.