Synapsin induce proliferation of T-cell lines against myelin basic protein obtained from rats with Experimental Autoimmune Encephalomyelitis

ALICIA LAURA DEGANO; ROTH, GERMAN ALFREDO

AUTOIMMUNITY

TAYLOR & FRANCIS LTD

Lugar: London; Año: 2009 p. 1 - 1

0891-6934

We have previously described that antibodies and T cells against myelin basic protein (MBP) rose under conditions to induceacute experimental autoimmune encephalomyelitis (EAE) bind other proteins present in the synaptosomal fraction, some ofthem identified as synapsin I. The aim of this study was to evaluate whether anti-MBP T-cell lines can be also activated bysynapsin. The analysis of rat anti-MBP T-cell lines cultured with each antigen showed that these cells responded also topurified rat synapsin and to the amino terminal portion of this protein. This recognition originated a proliferative responsewith a concomitant pattern of cytokine secretion similar to that induced by MBP itself implicating that this recognition wouldbe mediated by the T-cell receptor. On the other hand, anti-synapsin T-cell lines were not capable of responding to MBPstimulation. Therefore, the immunological cross-reactivity between both proteins occurs only in one direction and these crossreactivecells would be elicited only in animals sensitized with MBP. A possible implication of immunological agents againstMBP cross-reactive with extra-myelin proteins in the process of EAE is considered.