Natural flavonoids and calcium transport through biological membranes: molecular target and mechanisms of action?

ONTIVEROS, M; RINALDI, D.E; MARDER, M; ESPELT, M.V.; VIGIL, M.; MANGIALAVORI, I.; ROSSI, R.C; ROSSI, J.P.; FERREIRA GOMES, M

Lima

Congreso; Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level; 2019

Recently, there has been increasing interest in the research on natural flavonoids because of their beneficial properties for health [1]. Several studies have attributed them antioxidative, anti-inflammatory and anti-carcinogenic properties coupled with their capacity to modulate key cellular enzyme functions. Flavonoids exert their effect in different ways, in particular, there are flavonoids related with changes of intracellular calcium concentration suggesting that they could affect the calcium transport, including the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) and the plasma membrane Ca2+-ATPase (PMCA) [2-7]. However, the molecular mechanisms underlying this effect are not yetclear. This work seeks to investigate the effect of a flavonoid ?quercetin and gossypin- on PMCA4, a calmodulin regulated P-type ATPase responsible for the preservation of lowintracellular Ca2+ concentration in all the eukaryotic cells.[1] Erlund I, Silaste ML, Alfhan G, Rantala M & Aro A. (2002) Eur J Clin Nutr. 56, 891?898.[2] Mense SM, Hei TK, Ganju RK & Bhat HK. (2008) Environ Health Perspect 116, 426?433.[3] Syed DN, Khan N, Afaq F & Mukhtar H. (2007) Cancer Epidemiol Biomarkers Prev 16, 2193?2203.[4] Ramos S. (2007) J Nutr Biochem. 18, 427?442.[5] Wang I, Lin-Shiau S & Lin J. (1999). Eur J Cancer 35, 1517?1525[6] Ogunbayo OA, Harris RM, Waring RH, Kirk CJ, Michelangeli F. (2008) IUBMB Life. 60, 853-858.[7] Ogunbayo QA, Michelangeli F. (2014) FEBS J. 281, 766-777