Differential inhibition of Plasma Membrane Ca2+- ATPase by Quercetin and Gossypin

GENTILE,L.; RINALDI, D.E; MANGIALAVORI, I. .; MARDER, M; ROSSI, J.P.; FERREIRA GOMES, M

Cordoba

Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Flavonoids are commonly found in fruit and vegetables. Some flavonoids have been reported to reach micromolar concentration in human blood plasma. These compounds are related to health benefits, including antioxidant capacity and cancer chemoprotective properties. One mechanism proposed for the cancer protective effects, is that these flavonoids trigger the apoptosis of malignant cells through the Ca2+?dependent mitochondrial pathway, which can be activated through the elevation of cytosolic calcium. This could be due to that some flavonoids, like quercetin and gossypin inhibit Ca2+ transport systems, as the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) and the plasma membrane Ca2+-ATPase (PMCA).PMCA is a calmodulin regulated P-type ATPase responsible for the preservation of low intracellular Ca2+ concentration in all the eukaryotic cells. These compounds inhibit the SERCA, however, the effect of flavonoids on PMCA is not yet clear. The aim of this work was to explore the effect of flavonoids quercetin and gossypin on PMCA. Quercetin is a flavonol aglycone and gossypin is a flavonol glycoside with the linkage glycosidic in position 7. Also we study the effects of these compounds on the Ca2+ flux in HEK 293T cells and the Ca2+-ATPase activity in purified PMCA obtained from human erythrocytes.