PERSONAL DE APOYO
RINALDI debora Eugenia
congresos y reuniones científicas
Título:
Inhibition of the Plasma membrane Ca2+ -ATPase by Flavonoids
Autor/es:
RINALDI, D.E; MANGIALAVORI, I. .; ONTIVEROS,M; RIESCO, A; MARDER, M; ROSSI, J.P.; FERREIRA GOMES, M
Lugar:
Complejo Binacional Salto Grande
Reunión:
Congreso; Latin American Crosstalk in Biophysics and Physiology; 2015
Institución organizadora:
Latin American Crosstalk in Biophysics and Physiology
Resumen:
Flavonoids are commonly found in fruit and vegetables. Some flavonoids have been reported to reach micromolar concentration in human blood plasma [1]. These compounds would have cancer chemoprotective properties by triggering apoptosis via the Ca2+ dependent mitochondrial pathway which can be activated through an elevation of cytosolic calcium ([Ca2+]cyt). The increase of cytosolic [Ca2+] could be due to the fact that some flavonoids inhibit Ca2+ transport systems, as the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase and plasma membrane calcium ATPase (PMCA) [2-7]. PMCA is a calmodulin-regulated P-type ATPase responsible for the maintenance of low intracellular concentrations of Ca2+ in most eukaryotic cells (Figure 1). There are studies about how these compounds inhibit the SERCA (Figure 2, [8]), however, the effect of flavonoids on PMCA is not yet clear. The purpose of the present work was to explore the correlation between structure and activity of a wide range of flavonoids that could inhibit PMCA activity. Those flavonoids that were able to inhibit PMCA activity in more than 50% were studied in detail to determinate their mechanisms of action. They were: epigallocatechin 3-gallate (EGCg), quercetin and gossypin.