Concentration-dependant Effects of Allopregnanolone on Follicular Morphometric in Bovine Preantral Follicules

CARDONE, D. A.; CÁCERES, A. R. R.; SANHUEZA, M. A.; ROIG-CERDEÑO, M.; CUELLO CARRION, F. D.; LACONI, MYRIAM RAQUEL

Mendoza

Congreso; II Southern Science Conference 2024 - Virtual Conference and in Person Edition; 2024

Universidad de Mendoza, Argentina y Universidad de Vassouras, Brasil.

Allopregnanolone (ALLO) is a neurosteroid with potential roles in ovarian function, but its effects on bovine preantral follicles remain unexplored. In this study, we examined the concentration-dependent effects of ALLO on bovine preantral follicles cultured in vitro for 24 hours at concentrations ranging from 5 to 1000 nM. Follicular morphometry, including follicle, oocyte, and chromatin diameters, as well as follicular density and degeneration, were evaluated. Primordial follicle density did not exhibit significant alterations, but primary follicle density increased at 100 nM , while secondary follicle density augmented at 50 nM. Degeneration of primordial follicles increased at 100 nM and decreased at 1000 nM. In primary follicles, degeneration was significantly higher at 100 nM, 500 nM, and 1000 nM, whereas secondary follicle degeneration significantly increased at 10 nM. Morphometric analysis showed that primordial follicle diameters were reduced at 5 nM and 500 nM, but increased at 10 nM. Primary follicle diameters significantly decreased at 5 nM and 500 nM and increased at 100, while secondary follicle diameters were reduced at 50 nM, 100 nM , and 500 nM . Oocyte and chromatin diameters also showed significant reductions across different concentrations. These findings provide the first evidence of ALLO’s role in modulating the morphometry of bovine preantral follicles, suggesting its potential involvement in bovine reproductive physiology. Further research is needed to elucidate the molecular mechanisms underlying these effects and their implications for reproductive biotechnologies.