INVESTIGADORES
CUCHER Marcela Alejandra
congresos y reuniones científicas
Título:
Hydatid disease in genome era: comparative genomic analysis between Echinococuccus granulosus s.s. (G1 genotype) and Echinococcus canadensis (G7 genotype) draft genome
Autor/es:
MALDONADO L; ASSIS J; GOMES ARAUJO F; MACCHIAROLI N; CUCHER M; ROSENZVIT M; OLIVEIRA G; KAMENETZKY L
Reunión:
Congreso; 13th International Congress of Parasitology (ICOPA); 2014
Resumen:
BACKGROUND: Echinococcus canadensis is a platyhelminth parasite which keeps close phylogenetic relationship with Echinococcus granulosus and Echinococcus multilocularis members of the class Cestoda that are involved in hydatid infections of humans and animals. In South America three species of Echinococcus have been reported E. granulosus s.s. (G1 and G2 genotypes), E. canadensis (G6 and G7 genotypes) and E. ortleppi (G5 genotype). Only limited genetic information of E. canadensis G7  was reported so far. In this work we have sequenced the genome of this species. METHODS: High quality genomic DNA has been extracted and paired-end libraries have been sequenced by Illumina technology. Several pipelines of assembly have been evaluated. The genome has been de novo assembled with Velvet using different parameters until the best assembly was obtained. Also, reads have been mapped over E. multilocularis reference genome with BWA. Conserved genes have been annotated by CEGMA. RESULTS: Comparative studies have revealed high levels of nucleotidic identity between E. canadensis G7 with E. multilocularis as well as with E. granulosus s. s. G1.  Interestingly, the procedure for in silico annotation employed in this work allowed to identify more than 90% of highly conserved genes. CONCLUSIONS: This is the first report of E. canadensis G7 genome obtained by high throughput sequencing, allowing a broad genome view of this particular species.The knowledge of this new genome would provide information for comparative genomics allowing to adapt prevention and diagnosis tools to each epidemiological situation.