INVESTIGADORES
CUCHER Marcela Alejandra
congresos y reuniones científicas
Título:
Identification Of New Riboregulators In The Human Parasite Echinococcus granulosus.
Autor/es:
KAMENETZKY L; CUCHER M; PRADA L; MACCHIAROLI N; ROSENZVIT M
Reunión:
Simposio; Symposium Gene Expression and RNA Processing; 2011
Resumen:
Identification Of New Riboregulators In The Human Parasite Echinococcus granulosus Kamenetzky L, Cucher M, Prada L, Macchiaroli N, Rosenzvit M. In bilaterian animals, such as humans, flies and worms, hundreds of small RNAs, some conserved throughout bilaterian evolution, collectively regulate gene expression. In addition to microRNAs (miRNAs), other bilaterian small RNAs known as Piwi-interactig RNAs (piRNAs) seem to protect the genome regulating transposon expression. Here we obtained small RNAs libraries from the flatworm cestode Echinococcus granulosus, the causative agent of human hydatid disease. A subset of 283 RNA sequences ranging from 19 to 45 nucleotides were analyzed. Among them 185 were classified as miRNAs (Cucher et al., 2011). The remaining sequences with no rRNA/tRNA/mRNA hits were exhaustively analyzed and 24 were classified as putative piRNAs. Among them, 15 sequences (~ 60%) resembled bilaterian conservative piRNAs. Also they mapped to repetitive genome loci and/or have an adenine in position 10, like previously reported piRNAs . Interestingly, we found putative piRNAs homologous to human germline cells specific piRNAs (Girard et al., 2006). Additionally, we searched for piRNA biogenesis machinery components in the recently generated Echinococcus genomes and found the Piwi proteins orthologs represented in both E. granulosus and E. multilocularis species. This is the first report of the presence of piRNA-like small RNAs in cestodes. High throughput small RNA sequencing of periodate treated libraries are being designed to confirm these results. We show these findings in the context of the biological source of small RNAs: a parasite stage which has the ability to develop towards two drastically different developmental directions, sexual adult worm or asexual metacestode depending on the host environment.