INVESTIGADORES
CUCHER Marcela Alejandra
congresos y reuniones científicas
Título:
EFFECT OF PARASITE AND HOST FACTORS ON TAENIA CRASSICEPS ASEXUAL MULTIPLICATION, GROWTH AND SURVIVAL
Autor/es:
GARCIA L; PEREZ M; ANCAROLA ME; ROSENZVIT M; CUCHER M
Reunión:
Congreso; XI Congreso de la Sociedad Argentina de Protozoología; 2022
Resumen:
The zoonoses caused by cestode parasites are associated with poverty and deficient hygiene practices. In particular, cysticercosis is a neglected tropical disease caused by the metacestode larval stage (cysticercus) of Taenia solium and according to the WHO is one of the candidate infectious diseases to be eliminated by 2030. The most severe manifestation of the disease, called neurocysticercosis, is produced when cysticerci establish in the nervous system. Our aim was to identify the optimal in vitro culture conditions for the growth and asexual multiplication of T. crassiceps cysticerci, an experimental model for the study of cysticercosis.For this, we incubated cysticerci in different media with antibiotics: DMEM (A), DMEM + 10% FBS (B), DMEM + 10% FBS + excretion/secretion products of a hepatoma cell line (C) and DMEM + 10% FBS + excretion/secretion products of a lung carcinoma cell line (D). Also, we analysed the effect of parasite density by incubating 1 (1C) and 10 (10C) cysticerci per well. Number of buddings and parasite diameter were measured. Vitality was determined by trypan blue staining and morphology analysis.In 1C cultures only medium C produced a significant increase in diameter (P < 0.01), while in 10C cultures this effect was observed with all media (P < 0.001 and P < 0.05). In 1C and 10C, the highest increase in budding number was obtained with medium C (6.3-fold and 3.9-fold, respectively). Interestingly, in absence of host stimulus (medium A), 10C cultures produced a higher number of buds than 1C cultures. In the presence of medium B, heterogeneous results were obtained in all the conditions. Finally, parasites from cultures 1C and 10C showed high vitality in media C and D compared to the other media. These results show that both parasite density and culture medium influence the growth, asexual multiplication and survival of T. crassiceps in vitro and set the basis for the long-term in vitro culture of this T.solium model.