INVESTIGADORES
CLEMENTZ Adriana Laura
artículos
Título:
Use of discards of bovine bone, yeast and carrots for producing second generation bio-ethanol
Autor/es:
CLEMENTZ ADRIANA; MANUALE DEBORA; SANCHEZ ESTEBAN; VERA CARLOS; YORI JUAN CARLOS
Revista:
Biocatalysis and Agricultural Biotechnology
Editorial:
Department of Agriculture (USDA), Agricultural Research Service (ARS)
Referencias:
Año: 2019
ISSN:
1878-8181
Resumen:
The reutilization of agroindustrial wastes for the production of new products is an attractive option for reducingenvironmental pollution, generating value-added products and decreasing costs. Slaughterhouse discard bovine bone,brewery discard yeast and carrot discards from the Santa Fe province (Argentina) were used for producing ethanol.Bovine bone and yeast provided the support and active phase for fermentation biocatalysts while carrot discards werea sugar source. Discard bovine bone was collected directly from a slaughterhouse and then subjected to extractionwith acetone for removing lipids and blood traces. Both uncalcined and heat-treated bovine bone (calcined for 2 h at400, 600 and 800°C) were studied. Uncalcined bovine bone had a suitable pore structure for yeast immobilization anda suitable mechanical resistance for being used in stirred tanks. Two different strains of Saccharomyces cerevisiaewere immobilized and tested. One was a brewery yeast discarded from a local beer brewery and the other one acommercial yeast. The biocatalysts thus obtained were subjected to a thermal treatment at 35 ºC for 72 h in order todehydrate cells and form adhesins. The activity of the biocatalysts was tested in the fermentation of carrot discards.The maximum concentration of the obtained ethanol was 11.98 g L-1. It was possible to reuse the biocatalysts for 9reaction cycles. The possibility of regenerating the biocatalyst bone support by ultrasound is an advantage of this kindof material in comparison to common gel supports.