INSOLUBLE COMPLEX FORMATION BETWEEN ALPHA-AMYLASE FROM Aspergillus oryzae AND POLYACRYLIC ACID OF DIFFERENT MOLECULAR WEIGHT.

PORFIRI, MA. CECIIA; PICÓ, GUILLERMO; FARRUGGIA, BEATRIZ; ROMANINI, DIANA

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Lugar: Nancy; Año: 2010 vol. 45 p. 1753 - 1756

1359-5113

The insoluble complex formation between alpha-amylase and the strong anionic polyelectrolyte polyacrylic acid was studied by using turbidimetric and enzymatic activity. The highest molecular weight polyacrylic acid (100,000 and 240,000) proved to be suitable precipitating agents. They were insoluble at pH lower than 4-5, with a stoichiometric ratio polymer mol per protein mol of 1:52 and 1: 154, respectively. Electrostatic interactions are not the only factor in the formation of insoluble complexes. High percentage of alpha-amylase enzymatic activity maintains throughout time, even in the presence of polyelectrolyte. The application of precipitation conditions found when applying a bovine homogenate showed that it is not suitable for purification even if it proved to be useful methodology for the concentration of the enzyme and can be used as a first step of purification.