INVESTIGADORES
SOUTO cintia Paola
congresos y reuniones científicas
Título:
DNA barcoding the plants of Monte Desert, Argentina
Autor/es:
M. TADEY; C. P. SOUTO
Lugar:
Guelph
Reunión:
Congreso; Scientific abstracts from the 6th International Barcode of Life Conference; 2015
Institución organizadora:
University of Guelph
Resumen:
Background: Biodiversity conservation is a continuing challenge giventhe increasing habitat loss due to human activities causing extinction,local extinction, or reduction of species populations. Preserving speciesevolutionary potential (i.e., genetic variability) is important because it allows species to respond to changing environments, and therefore improve persistence of populations. DNA barcoding to determine the variation in species attributable to evolutionary history and the assessments of floristic surveys from geographic areas can enhance the development of regional barcoding libraries, providing vital data for community phylogeny construction and studies in ecology and conservation biology.Monte Desert occupies a vast area of Argentina and its being threatenedby human land use. This study aims to investigate the utility and speciesresolution capability of DNA barcoding in the vegetation of the MonteDesert.Weused a phylogenetic method (neighbour-joining trees) ofDNAbarcodes rbcLa and ITS2 to gain a better understanding of regional barcodevariation. Results: We analysed 75 species from 400 samples collectedthroughout Monte distribution. DNA barcodes were obtained for190 specimens and 61 species. We obtained ITS2 sequences from 48 specimens(27 species, 22 genera, 13 families) and rbcLa sequences from76 specimens (36 species, 29 genera, 17 families). Barcode analysis showedmean within-species divergences of 10.84% ± 0.74% and 10.32% ± 0.8%,16.32% ± 0.64% and 10.32% ±0.6% within genus, and 24.99% ± 0.17% and11.2% ± 0.2% within families for ITS2 and rbcLa, respectively. The meanintra-specific divergence was 9.07% ± 1.12% and 1.66% ± 0.19%, and themean species divergence from the nearest neighbour was 15.15% ± 0.43%and 1.93% ± 0.07% for ITS2 and rbcLa, respectively. Significance: Theseresults provide a better understanding of regional variation in barcodesequences in plants of Monte Desert, Argentina. This study expanded theglobal DNA barcode database for desert plants, and contributes to theknowledge of the application of barcoding to biogeography and conservationin plants.