Study of the addition of a freeze-dried probiotic strain isolated from kefir to poultry feed

ANA MORETTI; RAÚL GAMBA; JORGE PUPPO; NORBERTO MALO; ANDREA GÓMEZ-ZAVAGLIA; ÁNGELA LEÓN PELÁEZ; GRACIELA DE ANTONI; MARINA GOLOWCZYC

Workshop; International Workshop on Advances of Probiotics for Food and Veterinary Applications; 2016

The role of the microbiota in the gastrointestinal (GI) tract of animals for human consumption has been intensively studied due to its great significance in productivity, health, protection against pathogens, detoxification and modulation of the immune system. Antibiotic growth-promoters are used to increase animal performance and to control diseases. However, this practice is controversial due to health?s problems originated from the consumption of these products. Foods containing additives that promote optimal GI environment generate fewer problems in the animal development and may contribute to decrease GI infections by increasing beneficial bacteria (i.e. lactic acid bacteria) in the GI microbiota. Probiotics have also been claimed as alternatives to antibiotic growth-promoters. The aim of this study was to assess the viability of freeze-dried Lactobacillus plantarum CIDCA 83114 at feed mixture during storage time. We studied different carriers to incorporate this strain to feed: rice bran, whey milk and zeolite. The strain was incorporated in three concentrations: 10, 30 and 50% w/w. The mixture was stored for 40 days at room temperature and probiotic counts were performed on MRS agar at 0, 20 and 40 days of storage. Finally, feed added with L. plantarum CIDCA 83114 was evaluated for resistance to gastrointestinal conditions and aflatoxin B1 in an in vitro model. The best results of L. plantarum CIDCA 83114 viability and homogeneity were obtained with zeolite as a carrier. The viability of freeze-dried L. plantarum CIDCA 83114 added zeolite to 50 % w/w was not influenced by storage time with a count of 1 x 10 10 CFU/g at the end of storage. L. plantarum CIDCA 83114 was mixed with the zeolite (carrier), added to poultry feed at a concentration of 1% w/w and viability for 60 days of storage at room temperature was studied. Results showed that the concentration of L. plantarum CIDCA 83114 added to the poultry feed was 1 x 10 10 CFU/g at zero time (when it was incorporated to 50%) but decreasing during storage. L. plantarum CIDCA 83114 remained viable during passage through the GI tract either alone or mixed in poultry feed. We observed that L. plantarum CIDCA 83114 added to poultry feed using zeolite as a carrier showed the ability of aflatoxin B1 binding, which it was significantly higher compared to the controls.In conclusion, the viability of freeze-dried L. plantarum CIDCA 83114 in zeolite as a carrier is not influenced by storage time at room temperature. Nevertheless, when L. plantarum CIDCA 83114 is added to poultry feed, its viability decreases during storage time under non-controlled conditions. Moreover, L. plantarum CIDCA 83114 incorporated into the poultry feed maintains its viability during passage through the GI tract and demonstrate the ability of aflatoxin B1 binding. Thus, L. plantarum CIDCA 83114 has a potential application on poultry feed.