congresos y reuniones científicas
Cloning, expression and characterization of four soy allergens that cross react with bovine caseins
DOCENA, GUILLERMO HORACIO; CURCIARELLO, RENATA; CANDREVA ANGELA; GONZALEZ, VIRGINIA; BRUNO-BLANCH, LUIS; FOSSATI, CARLOS ALBERTO; PARISI, GUSTAVO; PETRUCCELLI, SILVANA
Congreso; 21º World Allergy Congress. WAC 2009; 2009
World Allergy Organization
Cow milk allergy constitutes the main food allergy observed in the pediatric population in Argentina and in many other countries. Besides, soy-based formulas are the nutritional substitute mostly employed as treatment in cow´s milk allergy. However, adverse reactions have been reported in 40% of the treated patients. Our group has found that four soy allergens (A5A4B3, Gly m alpha-conglycinin, Gly m Bd 30K or p34 and Gly m Bd 28K or p28) cross-react with bovine caseins. These proteins were obtained as recombinant polypeptides further used in different immunoenzimatic methods (immunoblotting, indirect and competitive ELISA). Employing polyclonal antisera, monoclonal antibodies and serum IgE antibodies specific to cow´s milk proteins we demonstrated that these components specifically cross react with casein proteins. Besides, a food allergy mouse model was used as an in vivo tool to confirm this cross reactivity. Employing different bioinformatics approaches we could model a common structure for these proteins, and then proposed three superficial epitopes that could be responsible for the observed reactivity. These peptides were synthetized on solid phase and reacted with different antibodies specific for bovine caseins. In conclusion, we detected cross-reactivity between allergens from cow´s milk and soy, and three potential immunodominant epitopes that could be the target in an attempt to reduce the allergenicity of soy components. The results obtained maybe the basis to understand the intolerance that cow´s milk allergic patients experience when soy-based formula are employed during the replacement treatment.