congresos y reuniones científicas
Cloning, expression and characterization of four soy allergens that cross react with bovine caseins
CURCIARELLO, RENATA; CANDREVA ANGELA; SMALDINI, PAOLA; GONZALEZ, VIRGINIA; BRUNO BLANCH, LUIS; PARISI, GUSTAVO; FOSSATI, CARLOS ALBERTO; PETRUCCELLI, SILVANA; DOCENA, GUILLERMO HORACIO
Viña del Mar
Congreso; 9º Congreso Latinoamericano de Inmunología (ALAI)- XII Chilean Society of Immunology Congress. LVII Argentinean Society of Immunology Satellite Scientific Meeting; 2009
Chilean Society of Immunology and Argentinean Society of Immunology
Cow´s milk allergy (CMA) constitutes the main food allergy observed in the pediatric population in Argentina and in many other countries. Although soy-based formulas are the nutritional substitute mostly employed for treatment of CMA, adverse reactions have been reported in 40% of the treated patients. Our group has previously described the presence of a cross reactivity between bovine caseins and the seeds globulin component A5A4B3, and other unknown residual cross reactive components. We also cloned, expressed and characterized a mutant fragment, called α-CTD, which would contain the possible immunodominant epitopes responsible of such cross-reactivity. The aim of this work was to obtain four recombinant soy allergens (A5A4B3, α subunit of β-conglycinin as the main component of 7S fraction, Gly m Bd 30K or p34 as the mayor allergic component, and Gly m Bd 28K or p28) to identify additional cross-reactive allergens using in vitro, in vivo and in silico methods.