INVESTIGADORES
PETRUCCELLI Silvana
artículos
Título:
Expression of the antibody 14D9 in Nicotiana tabacum hairy roots.
Autor/es:
MARTÍNEZ, CAROLINA;; PETRUCCELLI, SILVANA; GIULIETTI ANA MARÍA; ALVAREZ MARÍA ALEJANDRA
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Editorial:
Pontificia Universidad Católica de Valparaíso
Referencias:
Lugar: Valparaiso; Año: 2005 vol. 8 p. 170 - 176
ISSN:
0717-3458
Resumen:
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 ?g 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 ?g ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 ?g ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 ? g and 14 ?g respectively.