INVESTIGADORES
PETRUCCELLI Silvana
artículos
Título:
Transcription factor RF2a alters expression of the rice tungro Bacilliform virus promoter in transgenic tobacco plants.
Autor/es:
SILVANA PETRUCCELLI; DAI, S.; CARCAMO, R.; YIN, Y.; CHEN, S.; BEACHY, R. N.
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Editorial:
National Academy of Sciences of the United States of America
Referencias:
Lugar: University of California, Berkeley; Año: 2001 vol. 98 p. 7635 - 7640
ISSN:
0027-8424
Resumen:
The promoter from rice tungro bacilliform badnavirus (RTBV) is expressed only in phloem tissues in transgenic rice plants. RF2a, a b-Zip protein from rice, is known to bind to the Box II cis element near the TATA box of the promoter. Here, we report that the full-length RTBV promoter and a truncated fragment E of the promoter, comprising nucleotides -164 to +45, result in phloem-specific expression of beta-glucuronidase (GUS) reporter genes in transgenic tobacco plants. When a fusion gene comprising the cauliflower mosaic virus 35S promoter and RF2a cDNA was coexpressed with the GUS reporter genes, GUS activity was increased by 2-20-fold. The increase in GUS activity was positively correlated with the amount of RF2a, and the expression pattern of the RTBV promoter was altered from phloem-specific to constitutive. Constitutive expression of RF2a did not induce morphological changes in the transgenic plants. In contrast, constitutive overexpression of the b-ZIP domain of RF2a had a strong effect on the development of transgenic plants. These studies suggest that expression of the b-Zip domain can interfere with the function of homologues of RF2a that regulate development of tobacco plants.