INVESTIGADORES
PEREZ Pablo Fernando
congresos y reuniones científicas
Título:
Lactobacilli strains enhance human β defensin 2 (HBD-2) and mouse β defensin 3 (mBD-3) expression.
Autor/es:
HUGO, A. A.; SZYMANOWSKI, F. AND PÉREZ, P. F.
Reunión:
Otro; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología.; 2019
Resumen:
Human β defensin 2 (HBD-2) and mouse β defensin 3 (mBD-3) are ortholog antimicrobial peptides produced by enterocytes. Microbial signals can induce expression of both defensins via the NF-kB pathway. The aim of this study is to investigate the capacity of two lactobacilli strains (L. delbrueckii subsp lactis CIDCA 133 and L. delbrueckii subsp bulgaricus, CIDCA 331) to induce HBD-2 and mBD3 expression. Expression of HBD-2 was measured by qPCR in Caco-2/TC7 cells. Monolayers were incubated with lactobacilli and positive controls were stimulated with flagellin (FliC). NF-KB expression was assessed by using a reporter HT-29 cell line. Expression of mBD-3 was quantified by q-PCR in Balb-c mice. Lactobacilli were administrated in the drinking water. Intestinal samples were collected at day 1 and 7.Lactobacilli increased per se the HBD-2 expression in Caco-2/TC7 cells and had an additive effect when cells were incubated with FliC. Lactobacilli dowregulated the expression of NF-kB in FliC-stimulated HT-29 cells. In the murine model, lactobacilli upregulated the expression of mHBD-3 at day 1. Expression returned to basal levels at day 7.Results showed that lactobacilli enhanced the HBD-2 expression per se and potentiate FliC-induced expression. Lactobacilli also increased transiently the expression of mBD-3 in mice. However, lactobacilli down-regulated the NF-kB expression induced by FliC thus indicating that they are able to stimulate expression of antimicrobial peptides without further activation of the NF-kB response pathway.