INVESTIGADORES
PEREZ Pablo Fernando
congresos y reuniones científicas
Título:
Murine model of gastrointestinal infection with Bacillus cereus B10502
Autor/es:
ROLNY, I. S.; MINNAARD, J. AND PÉREZ, P. F.
Reunión:
Congreso; LXI Reunión Annual de la Sociedad Argentina de Inmunología; 2013
Resumen:
Bacillus cereus is a spore-forming microorganism able to produce intestinal and non-intestinal pathologies. In the context of intestinal illness there are two syndromes: emetic and diarrheic. Extracellular factors of the strain B10502 lead to cell damage in in vitro assays and vegetative cells adhered to enterocyte-like Caco-2 cells. In the present study we analyzed the effect of the infection of mice with vegetative B.cereus B10502. C57BL/6 female mice were administered intragastrically with 109 cfu/mouse. After different times post-infection (PI) mice were euthanized and samples from intestine, mesenteric lymph nodes (MLN) and faeces were analyzed. Tissues were analysed by haematoxylin-eosin (HE) and Alcian blue staining (goblet cells). B. cereus access to different tissues (stomach, duodenum, jejunum, ileum and cecum), faeces, Peyer´s patches (PP) and MLN were analyzed by inoculating samples in brain heart infusion supplemented with glucose 0.1% p/v (BHIG) and further plating on BHIG agar. Real-time PCR was used to determine CXCL2, TNF-α, IL-6, IL-10, IL-12p40 and INF-γ cytokines in PP and MLN. Infection of mice with B. cereus B10502 leads to an increase in the PP size. A significant (P