PERSONAL DE APOYO
FERNÁNDEZ Jorge GermÁn
congresos y reuniones científicas
Título:
PROTEOMICS OF THE CHLOROPLAST STROMA OF LOTUS JAPONICUS UNDER COLD STRESS
Autor/es:
CALZADILLA PI; VILAS JM; MAIALE; VALACCO MP; JORGE GERMÁN FERNÁNDEZ; MORENO S; RUIZ OA
Lugar:
Córdoba
Reunión:
Congreso; 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Low temperature is one of the most important factors affecting plant growth, directly altering the photosynthetic process and leading to photo-inhibition. With the aim to address the photosynthetic acclimation response of L. japonicus under cold stress, two ecotypes (MG-1 and MG-20) were studied. Previous data showed that photo-inhibition occurs in stress and differentially between ecotypes, being MG-1 more affected than MG-20. In this study, we used a proteomic approach to evaluate changes in the chloroplast stroma of both ecotypes after 24 h of low temperature. A total of 450 proteins were identified, with 325 uncharacterized in the L. japonicus protein database. Proteins were classified and 168 and 86 were differentially accumulated in MG-1 and MG-20, respectively, in the stress treatment compared to controls. Of these, only 3 and 4 proteins were more abundant, while 165 and 82 showed a reduction in its accumulation at low temperature. A functional annotation and classification was made by Gene Onthology, KEGG Metabolic Pathways and blastp. The results obtained showed that down-regulated proteins were mainly related to amino acid, carbohydrate and energy metabolisms. However, particular metabolic pathways were different regulated in each case and for each ecotype. Data obtained may be useful in identifying proteins with a key role in low temperature acclimation in legumes.