IN VITRO SECRETOMIC ANALYSIS OF PROSTATE CANCER CELLS AND BONE PROGENITOR CELLS GROWING IN CO-CULTURE SYSTEMS

NICOLAS ANSELMINO; ALEJANDRA PAEZ,; SOFIA LAGE VICKERS; COTIGNOLA, JAVIER H; MARIA PIA VALACCO; GERALDINE GUERON; ELBA VAZQUEZ.

CABA

Congreso; Reunion Conjunta de sociedades de Biociencias; 2017

Sociedad Argentina de Investigacion clinica

Currently our view of cancer has evolved to include, in additionto the transformed cells that have deregulated homeostatic mechanisms,a wide spectrum of cells of the tumor microenvironment. Thedialogue established between the tumor cells and its microenvironment,is an essential determinant of the characteristics of the tumorprogression. The dissemination profile of Prostate Cancer (PCa)shows tendency to develop in the bone, where tumoral cells interactwith the microenvironment disrupting the bone tissue balance. Thiswork aimed at analyzing, which soluble factors could be potentialmediators of the chemical cross-talk between PCa cells and boneprogenitor cells. For this purpose, we used co-culture transwell systemsof PC3 (human PCa cells) with the pre-osteoclastic Raw264.7or pre-osteoblastic MC3T3 cell lines (murine cells), where cellsshared the culture media (CMs) without physical contact for 24h.We employed mass spectrometry -nanoLC-MS/MS(Orbitrap)- forthe secretome analysis of the CMs. The obtained spectra were analyzedwith the Proteome Discover Software and compared with both,human and murine protein databases. Results highlight a differentialprofile of proteins released to the CM when tumor cells are grownin co-culture compared to controls. These results could potentiallyexplain the altered mRNA expression levels assessed by RTqPCRin PCa cells growing in-culture, displaying significant up-regulationfor HO-1 ANXA2, ANXA2R,OPG and PTHrP (p