INVESTIGADORES
MORENO Silvia Margarita
congresos y reuniones científicas
Título:
Differential roles of protein kinase A regulatory subunits in Mucor circinelloides
Autor/es:
OCAMPO J, MC CORMACK , MORENO S, GARRE V, ROSSI S
Lugar:
Potrero de los Funes- San Luis
Reunión:
Congreso; 47 Reunion Anual de la SAIB; 2011
Institución organizadora:
SAIB
Resumen:
PKA is a tetramer composed of two Regulatory (R) and two Catalytic subunits (C). We have demonstrated the existence of 4 genes for R and 10 genes for C in M. circinelloides. In a previous work we had disrupted the gene pkaR1 (DR1) and described its role in growth and morphology. DR1 showed a reduction in radial growth, alterations in germination rate, cell volume, germ tube length, and asexual sporulation. In the present work, we have disrupted the genes pkaR2 (DR2), pkaR3 (DR3), or pkaR4 (DR4) with the aim of analyzing if each PKAR isoform has a different role in the differentiation process. The germ tube emission in DR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inM. circinelloides. In a previous work we had disrupted the gene pkaR1 (DR1) and described its role in growth and morphology. DR1 showed a reduction in radial growth, alterations in germination rate, cell volume, germ tube length, and asexual sporulation. In the present work, we have disrupted the genes pkaR2 (DR2), pkaR3 (DR3), or pkaR4 (DR4) with the aim of analyzing if each PKAR isoform has a different role in the differentiation process. The germ tube emission in DR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inpkaR1 (DR1) and described its role in growth and morphology. DR1 showed a reduction in radial growth, alterations in germination rate, cell volume, germ tube length, and asexual sporulation. In the present work, we have disrupted the genes pkaR2 (DR2), pkaR3 (DR3), or pkaR4 (DR4) with the aim of analyzing if each PKAR isoform has a different role in the differentiation process. The germ tube emission in DR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDR1 showed a reduction in radial growth, alterations in germination rate, cell volume, germ tube length, and asexual sporulation. In the present work, we have disrupted the genes pkaR2 (DR2), pkaR3 (DR3), or pkaR4 (DR4) with the aim of analyzing if each PKAR isoform has a different role in the differentiation process. The germ tube emission in DR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inpkaR2 (DR2), pkaR3 (DR3), or pkaR4 (DR4) with the aim of analyzing if each PKAR isoform has a different role in the differentiation process. The germ tube emission in DR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDR2 is earlier than in the wild-type strain. PKAR4 appears to be essential for the fungus survival. The homokaryotic DR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDR4 strain was not stable; with the successive cultures it became heterokaryotic. DR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDR4 strain had an impediment to emit the germ tube under aerobic conditions and after the transition from anaerobic to aerobic conditions; it also has an increased cell volume. DR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDR3 shows slight differences from wt. The expression of the pkaR genes was assessed in Dr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role inDr1, DR2 and DR3 by semiquantitative RT-PCR. Different mRNA expression was detected in the knockout strains for each pkaR gene. The results indicate that each PKAR isoform has a different role in M. circinelloides physiology, thus contributing to the specificity of cAMP-PKA pathway.physiology, thus contributing to the specificity of cAMP-PKA pathway.