Evidences of -1,3-glucans in Mucor rouxii

PEREYRA, E., MORENO, S.

Rosario, Santa Fe, Argentina

Congreso; 42 Reunion Anual SAIB; 2006

SAIB

We have cloned and characterized Rho 1 protein from Mucor rouxii. The small GTP binding protein have been shown to be the regulatory protein of the â 1,3 glucan synthase complex, in fungi in which â 1,3 glucans are major components of the cell wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. in which â 1,3 glucans are major components of the cell wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. â 1,3 glucan synthase complex, in fungi in which â 1,3 glucans are major components of the cell wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. â 1,3 glucans are major components of the cell wall. Even though glucans have not been detected in zygomycetes, caspofungin, an echinochandin known to be an inhibitor of â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. â 1,3 glucan synthase complex, is shown here to have a negative effect on growth and to produce an alteration on morphology when added to M. rouxii growth culture medium. The minimum effective concentration (MEC) was 10 µ g/ml and the fungus produced short, stubby and highly branched hyphae. Localization in germlings was visualized by immunofluorescence, the protein shows to be localized in patches in the mother cell surface and excluded from the germ tubes. Similar results are obtained by using a specific monoclonal antibody anti-â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. â 1,3 glucans. The sresults suggest that Muicor rouxii have â 1,3 glucans in the cell wall. wall. â 1,3 glucans in the cell wall.