Physiological and proteomic response of Escherichia coli O157:H7 to a bioprotective lactic acid bacterium in a meat environment

ORIHUEL ALEJANDRA; TERAN LUCRECIA; RENAUT JENNY; PLANCHON SEBASTIEN; VALACCO MARIA PIA; MASIAS EMILSE; MINAHK CARLOS; VIGNOLO GRACIELA; MORENO SILVIA; ALMEIDA ANDRE M. DE; SAAVEDRA LUCILA; FADDA SILVINA

FOOD RESEARCH INTERNATIONAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019 vol. 125 p. 108622 - 108627

0963-9969

The enterohemorrhagic Escherichia (E.) coli (EHEC) is a pathogen of great concern for public health and the meatindustry all over the world. The high economic losses in meat industry and the high costs of the illness highlightthe necessity of additional efforts to control this pathogen. Previous studies have demonstrated the inhibitoryactivity of Enterococcus mundtii CRL35 towards EHEC, showing a specific proteomic response during the coculture.In the present work, additional studies of the EHEC-Ent. mundtii interaction were carried out: i) differentialprotein expression of E. coli O157:H7 NCTC12900 growing in co-culture with Ent. mundtii in a meatenvironment, ii) the reciprocal influence between these two microorganisms in the adhesion to extracellularmatrix (ECM) proteins and iii) the possible induction of the phage W933, coding for Shiga toxin (Stx1), by Ent.mundtii CRL35. Proteomic analysis showed a significant repression of a number of E. coli NCTC12900 proteins inco-culture respect to its single culture, these mostly related to the metabolism and transport of amino acids andnucleotides. On the other hand, statistically significant overexpression of EHEC proteins involved in stress,energy production, amino acid metabolism and transcription was observed at 30 h respect to 6 h when EHECgrew in co-culture. Data are available via ProteomeXchange with identifier PXD014588. Besides, EHEC showed adecreased adhesion capacity to ECM proteins in the presence of the bioprotective strain. Finally, Ent. mundtiiCRL35 did not induce the lytic cycle of W933 bacteriophage, thus indicating its potential safe use for eliminatingthis pathogen. Overall, this study expands the knowledge of EHEC- Ent. mundtii CRL35 interaction in a meatenvironment, which will certainly contribute to find out effective biological strategies to eliminate this pathogen