INVESTIGADORES
MORENO Silvia Margarita
artículos
Título:
p8/nupr1 regulates DNA-repair activity after double strand gamma irradiation-induced DNA damage
Autor/es:
GIRONELLA M; MALICET C; CANO C; SANDI M; HAMIDI T; NEM TAUIL R; BASTON M; VALCCO P; MORENO S; LOPEZ F; NEIRA JL; DAGORN JC; IOVANNA JL
Revista:
JOURNAL OF CELLULAR PHYSIOLOGY
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Año: 2009 vol. 221 p. 594 - 602
ISSN:
0021-9541
Resumen:
The stress protein p8 is a small, highly basic, unfolded and multifunctional protein. We have previously shown that most of its functions are exerted through interactionswith other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanism, by which p8 binds and negatively regulates Msl1, a HAT-associated protein, which in turn binds the DNA-damage associated Tp53BP1 protein to facilitate DNA-repair following DNA ã-irradiation. Contrary to the HAT-associated activity, Msl1-dependent DNA-repair activity is almost completely dependent of Tp53BP1 expression. The picture that has emergedfrom our findings is that Tp53BP1 could be a scaffold that gets the HAT Msl1-dependent DNA-repair activity to the sites of DNA damage. Finally, we also found that, although p8 expression is transiently activated after ã-irradiation, it is eventually submitted to sustained down-regulation, presumably to allow velopment of Msl1-associated DNA-repair activity. We conclude that interaction of Msl1 with Tp53BP1 brings Msl1-dependent HAT activity to the vicinity of damaged DNA.