INVESTIGADORES
CASAIS marilina
congresos y reuniones científicas
Título:
Progesterone effect on factors involved in the progression of endometriosis
Autor/es:
SERÁN C; VALLCANERAS S; PAOLETTI GEUNA G; DELSOUC MB; CASAIS M
Lugar:
San Luis
Reunión:
Congreso; XXXVII Reunión Anual de la Sociedad de Biología de Cuyo; 2019
Resumen:
Endometriosis (EDT) is a hormone-dependent inflammatory disease characterized by the implantation and growth of endometrium-like tissue outside the uterine cavity. It is known that estradiol has an unfavorable effect in the development of pathology, while progesterone role is less established. Therefore, the aim of this work was to study the influence of progesterone on factors related to apoptosis, invasion and inflammation that are involved in the development of endometriotic lesions in a murine model. The EDT was surgically induced in C57BL/6 mice by autotransplanting 3 pieces of right uterine horn to the intestinal mesentery. From day 1 after the operation, the animals of the control group (n= 7) received sterile corn oil, while the animals of the experimental group (n= 7) received 2.5 mg Progesterone/kg bw/day subcutaneously for 1 month. Then, the mice were sacrificed by cervical dislocation and the lesions were removed and stored at -80 °C for subsequent gene expression analysis (RT-PCR) of markers of the intrinsic apoptotic pathway: Bax and Bcl-2, inflammation markers: IL-1β and TNF-α, and TNF receptors (p55 and p75). Peritoneal fluid was also collected for the determination of the enzymatic activity of metalloproteinase 2 (MMP-2) and its zymogen by zymography. The data were statistically analysed by the Student t test (p