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Título:
Influence of gonadotropin-releasing hormone antagonist, cetrorelix, from coeliac ganglion on the ovarian estradiol and nitric oxide release
Autor/es:
MORALES LD; VALLCANERAS S; CASAIS M
Reunión:
Congreso; Sociedad de Biología de Cuyo XXXIV Reunión Científica Anual; 2016
Resumen:
INFLUENCE OF GONADOTROPIN-RELEASING HORMONE ANTAGONIST, CETRORELIX, FROM COELIAC GANGLION ON THE OVARIAN ESTRADIOL AND NITRIC OXIDE RELEASEMorales LD, Vallcaneras S, Casais M. Lab Biol Reprod (LABIR). FQByF-UNSL, IMIBIO-SL CONICET, San Luis. E-mail: lauradanimorales@hotmail.comThere are precedents that demonstrate the extrahypothalamic origin of gonadotropin-releasing hormone (GnRH) as well as its inhibitory effect on ovarian steroidogenesis. Taking into account this information, the aims of this work were to study the effect of a GnRH antagonist on: 1) the ovarian release of estradiol (E2) and nitric oxide (NO), 2) the ovarian gene expression of P450 aromatase (P450arom, E2 synthesis enzyme), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) on day 21 of pregnancy in the rat. We used the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system. The system was incubated in Krebs Ringer at 37ºC, keeping CG and O connected by the SON, in separate compartments. The GnRH antagonist: cetrorelix 10-6 M (Ctx) was added in the ganglion compartment. E2 was determined by RIA and NO by the Griess technique in the ovarian compartment at 30 and 180 min of incubation. Ovarian gene expressions of P450arom, eNOS and iNOS were assessed by RT-PCR at 180min. One-way ANOVA and Tukey test were used (p