Androstenedione in Superior Mesenteric Ganglion Increase the Release and Metabolism of Ovarian Progesterone in Estrus. Participation of Nitric Oxide and Norepinephrine

GIL ROSAS M; VEGA OROZCO A; CASAIS M

Congreso; Sociedad Argentina de Investigación Clínica (SAIC); 2016

Androstenedione in Superior Mesenteric Ganglion Increase the Release and Metabolism of Ovarian Progesterone in Estrus. Participation of Nitric Oxide and Norepinephrine.Gil Rosas M, Vega Orozco A, Casais M.Laboratorio de Biologia de la Reproducción (LABIR). FQBF-UNSL, IMIBIO-SL, CONICET. San Luis. Argentina. Corpus Luteum (CL) is an endocrine secretory gland whose main product is progesterone (P4), which is essential for maintenance during the estrous cycle. Previous works have shown that peripheral sympathetic ganglia have steroid receptors which are able to cause changes in ovarian steroidogenesis. However, at present little is known about the role of androstenedione (A2) in Superior Mesenteric Ganglion (SMG) on the formation and maintenance of CL in oestrus. For this reason, the objectives were to demonstrate whether A2 in SMG modifies in ovary: 1) the P4 release 2) the gene expression of 3 β-HSD (RNAm and protein) and 20α-HSD (P4 synthesis and degradation respectively); and 3) whether such modifications are related with changes on NO, norepinephrine (NE) and on the expression levels of apoptosis markers such as Bax/Bcl-2, Fas/Fas-L and eNOS/ iNOS. The ex vivo SMG-Ovarian Nervous Plexus(ONP)-Ovary system was incubated with A2 added in ganglion. For this, we used a cuvette with two compartments with Krebs Ringer solution, pH 7.4, in a metabolic bath at 37°C. P4 (RIA), Nitrite (Griess method) and NE (HPLC) were determined at 15, 30, 60 and 120 min. The gene expression by RT-PCR at 120 min and protein levels by Western Blot. ANOVA-1 and Tukey test (p