The circadian oscillation of clock factor BMAL1 is modified by TNFRP55 deficiency at the end of pregnancy

DE LA VEGA M; ANZULOVICH AC; CASAIS M

Congreso; 3ra Reunión Conjunta de Sociedades de Biología de la República Argentina. 9-11/09 San Miguel de Tucumán; 2015

THE CIRCADIAN OSCILLATION OF CLOCK FACTOR BMAL1 IS MODIFIED BY TNFRP55 DEFICIENCY AT THE END OF PREGNANCYDe la Vega M, Anzulovich AC, Casais M. Lab Biol Reprod (LABIR)-FQByF-UNSL, IMIBIO-SL-CONICET - San Luis. E-mail: magalidlv0@gmail.com.ar Previously, we demonstrated that progesterone (P4) and the proteins levels of its synthetic enzyme, 3β-HSD, display circadian rhythmicity in the ovary at the end of pregnancy, and that lack of p55 TNF receptor overrides this rhythm both P4 and 3β-HSD. Due to the presence of elements response to clock genes in the regulatory region of the gene of 3β-HSD, we wanted to investigate whether transcription factor that is part of the clock circuit upregulation clock machinery, Bmal1 presents circadian variation and if the absence of TNFRp55 affects their temporal expression in the ovary at the end of pregnancy. Wild type and TNFRp55-/- mice, were maintained on a 12h light: 12h dark cycle, at 24±2°C, with food and water ad libitum. Five days before the experiment, mice were kept under constant darkness conditions. Ovaries were isolated every 6 h during a 24h period. Bmal1 protein levels were determined by Western blot. As expected, Bmal1 protein levels display a circadian oscillation in the ovary at the end of pregnancy (p