NORADRENERGIC INFLUENCE ON THE OVARY: GnRH RELEASE AND ITS INVOLVEMENT IN OVARIAN APOPTOTIC MECHANISMS

BRONZI CD; RODRIGUEZ D; VEGA OROZCO A; RASTRILLA AM; SOSA Z; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXI Reunión Científica Anual; 2013

NORADRENERGIC INFLUENCE ON THE OVARY: GnRH RELEASE AND ITS INVOLVEMENT IN OVARIAN APOPTOTIC MECHANISMS Bronzi CD, Rodriguez D, Vega Orozco A, Rastrilla AM, Sosa Z, Casais M. Lab. Biol. Reprod (LABIR)- FQByF -UNSL, IMIBIO-SL CONICET, San Luis. E-mail: cdbronzi@hotmail.com The Gonadotropin Releasing Hormone (GnRH) seems to be an important intraovarian regulator. The aim of this study was to evaluate if noradrenaline (NA) modulates the GnRH, progesterone (P4), and Oestradiol (E2) release, the expression of synthesis and degradation enzymes of P4 (3β-HSD and 20α-HSD, respectively), and the expression of apoptotic bax and antiapoptotic bcl-2 factors, in the ovary of rat at diestrus II. The ovary was incubated in Krebs Ringer buffer at 37ºC with and without the addition of NA (10-6M). Extractions of the ovarian incubation liquid were carried out at 60 and 120 min. P4, E2 and GnRH were measured by RIA, and 3β-HSD, 20α-HSD, bax and bcl-2 mRNA expressions by RT-PCR. ANOVA 1 followed by Bonferroni test for statistical significance was used. NA in ovary increased the liberation of GnRH (p