Noradrenaline in coeliac ganglion favors the corpus luteum survival through an indirect effect

BRONZI CD; RODRÍGUEZ D; VALLCANERAS S; SOSA ZY; RASTRILLA AM; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXI Reunión Científica Anual; 2013

NORADRENALINE IN COELIAC GANGLION FAVORS THE CORPUS LUTEUM SURVIVAL THROUGH AN INDIRECT EFFECT Bronzi CD, Rodríguez D, Vallcaneras S, Sosa ZY, Rastrilla AM, Casais M. Lab Biol Reprod (LABIR)-FQByF -UNSL, IMIBIO-SL CONICET, San Luis. Email: cdbronzi@gmail.com It is known that the ovary is innervated by superior ovarian nerve (SON) whose fibers originate in the Coeliac Ganglion (CG). The objective of this study was to evaluate if Noradrenaline (NA), added in the CG, modulates the release of GnRH, Progesterone (P4) and Oestradiol (E2), the enzyme expressions of P4 synthesis (3β-HSD) and degradation (20α-HSD), and the apoptotic bax and anti-apoptotic bcl-2 factors, in the ovary of rat at Diestrous II stage. The CG-SON-ovary system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary, connected by the SON, in separate compartments. CG was incubated with (control group) and without NA (10-6M). Extractions of the ovarian incubation liquids were carried out at 60 and 120 min. P4, E2 and GnRH were measured by RIA and the expression of 3β-HSD, 20α-HSD, bax and bcl-2 by RT-PCR. ANOVA 1 followed by Bonferroni test with a statistical significance of (p