Estradiol stimulates ovaric GnRH liberation through a direct and indirect effect from the celiac ganglion

BRONZI D; DANERI C; VALCANERAS S; CASAIS M; SOSA Z; RASTRILLA AM

Congreso; Sociedad de Biología de Cuyo XXX Reunión Científica Anual. San Luis; 2012

ESTRADIOL STIMULATES OVARIC GnRH LIBERATION THROUGH A DIRECT AND INDIRECT EFFECT FROM THE CG. Bronzi D, Daneri C, Valcaneras S, Casais M, Sosa Z. Rastrilla AM. LABIR. U.N.S.L. zsosa@unsl.edu.ar Estradiol (E2) is involved in the regulation of reproductive processes, acting on peripheral organs and sympathetic neurons associated with reproduction. The objective was to evaluate if E2 added in coeliac ganglion (CG) and in ovary alone modulates the ovarian release of GnRH and steroidogenic hormones in DII. We used two experimental schemes: a)- the CG-superior ovarian nerve (SON)-ovary system with and without the presence of E2 (10-8M) in ganglion compartment and b)- incubations of ovary alone with and without the presence of E2 (10-8M). The systems in both case were incubated in Krebs Ringer at 37ºC, in specific cuvettes designed for this purpose. Periodic extractions of the ovarian incubation liquid were carried out at 60 and 120 min. A2 , E2 and GnRH and were measured by RIA. ANOVA 1 followed by Tuckey test with a statistical significance of p