Acetylcholine in coeliac ganglion modifies ovary regression in diestrous

BRONZI D; DANERI C; VEGA O A; SOSA Z; RASTRILLA AM; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXX Reunión Científica Anual. San Luis; 2012

ACETYLCHOLINE IN COELIAC GANGLION MODIFIES OVARY REGRESSION IN DIESTROUS Bronzi D, Daneri C, Vega O A, Sosa Z, Rastrilla AM, Casais M. Laboratorio de Biología de la Reproducción. U.N.S.L. zsosa@unsl.edu.ar Acetylcholine (Ach) is the major sympathetic preganglionic neurotransmitter. Using the ex vivo coeliac ganglion (CG)-superior ovarian nerve (SON)-ovary system, the aims were to investigate whether Ach in CG modulates: 1)the ovarian release of Progesterone (P4) and the expression of 3β-HSD and 20α-HSD, 2) the ovarian release of Noradrenaline (NA) and dopamine (DA); 3) the expression of bax and bcl2. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. Ach (10-6M) was added in the ganglion compartment in the experimental group. Periodic extractions of the ovarian incubation liquid were carried out at 60 and 120 min and ovaries were isolated at 120 min. P4 levels were determined by RIA and catecholamines by HPLC. 3β-HSD, 20α-HSD, bax and bcl2 transcripts levels were measured by RT-PCR. ANOVA 1 followed by Tuckey test with a statistical significance of p