Effect of progesterone on luteal regression via a neural pathway in late pregnant rat

GHERSA F; VALLCANERAS S; CASAIS M; RASTRILLA AM

Congreso; Sociedad de Biología de Cuyo XXX Reunión Científica Anual. San Luis; 2012

EFFECT OF PROGESTERONE ON LUTEAL REGRESSION VIA A NEURAL PATHWAY IN LATE PREGNANT RAT. Ghersa F, Vallcaneras S, Casais M, Rastrilla AM. Lab. Biol. Reprod (LABIR)- FQByF -UNSL - San Luis -Chacabuco 917 ssvallca@unsl.edu.ar Previously, we demonstrated that Progesterone (Pg) added to the coeliac ganglion (CG), via the superior ovarian nerve (SON), is able to stimulate the ovarian Pg release. The object of this study was to analyze the effect of Pg, through SON, on the functional and structural luteal regression of 21 pregnant rats using the ex vivo CG-SON-ovary system. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. Pg (10-5M) was added in the ganglion compartment [(Pg)g], Controls were not stimulated. Periodic extractions of the ovarian incubation liquid were carried out at 30, 60, 120 and 180 min. To corroborate previous results of Pg, it was determined by RIA. At 180 min, the luteal mRNA expression of 3β-HSD, 20α-HSD (synthesis and degradation enzymes respectively), Fas and Fas-L were analysed by RT-PCR. ANOVA 1 followed by Tuckey test with a statistical significance of p