Influence of estradiol in the peripheral neural regulation of ovary at the end of pregnancy in the rat

CAMPO VERDE ARBOCCO F; VALLCANERAS S; CASAIS M; RASTRILLA AM

Mendoza

Congreso; Sociedad de Biología de Cuyo XXVI Reunión Científica Anual; 2008

Influence of estradiol in the peripheral neural regulation of ovary at the end of pregnancy in the rat Campo Verde Arbocco F, Vallcaneras S,  Casais M, Rastrilla AM Laboratorio de Biología de la Reproducción-FQByF-UNSL Chacabuco 917. email: arboccocv@hotmail.com Our research group have standardized an integrated ex vivo Celiac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-Ovary) system, whose vitality has been proven physiologically and histologically. The objective was to research if estradiol (E2) in CG modifies the physiology of the ovary at the end of pregnancy (day 21) in the rat. The CG-SON-Ovary system was incubated in Krebs Ringer-glucose-albumin (0.1 mg/ml) at 37 ºC, keeping CG and ovary connected by the SON, in separate cuvettes. In the CG compartment was added E2 in concentrations 10-6M, 10-8M and 10-9M. Controls were not stimulated. In the ovary compartment, at 30, 60, 120, 180 and 240 min of incubation,  Progesterone (P) liberated was measured by RIA and nitric oxide (NO) by Griess reaction; at the end of the incubation period (240 min), in the luteal tissue was determined the enzymatic activity of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-HSD (synthesis and degradation of P enzymes, respectively). Was utilized  ANOVA 1 followed by Tuckey test with a statistical significance of p <0.05. The results were: E2 in CG, decreased the release of ovarian P in all concentrations tested; did not produce changes in the release of NO neither in the enzymatic activities. Estradiol in CG, through NOS, acts in favor of functional luteal regression.