Estradiol in celiac ganglion, modulates the luteal regression at the end of pregnancy in rat.

CAMPO VERDE ARBOCCO F; VALLCANERAS SS; HAPON B; CASAIS M; RASTRILLA AM

San Luis

Congreso; Sociedad de Biología de Cuyo XXVII Reunión Científica Anual; 2009

Estradiol in celiac ganglion, modulates the luteal regression at the end of pregnancy in ratCampo Verde Arbocco F, *Vallcaneras S, *Hapón B, *Casais M, Rastrilla AM. Lab Biología de la Reproducción-FQByF-UNSL. *CONICET. San Luis. Email: mcasais@unsl.edu.ar Estradiol (E2) is a key hormone in the regulation of reproductive processes acting both on peripheral organs and sympathetic neurons associated to reproductive function. We found, using the ex vivo Celiac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-Ovary) system, that E2 added to the CG inhibited the liberation of progesterone (P) in ovary but did not modify the activity of 3β-hydroxysteroid dehydrogenase (3βHSD), and 20αHSD (synthesis and degradation P enzymes, respectively). The objective of this work was to research, in the same experimental scheme, if E2 in CG modifies the luteal regression. The ex vivo system was incubated in metabolic bath, keeping CG and ovary connected by the SON, in separate cuvettes. In CG compartment E2 was added in 10-6, 10-8 and 10-9M concentrations. Controls were not stimulated. After 240 min of incubation, RNA was extracted from luteal tissue to determine the expression of 3βHSD, 20αHSD, Bcl-2 (antiapoptotic) and Bax (proapoptotic) genes by RT-PCR. Was utilized ANOVA 1 followed by Tuckey test with a statistical significance of p