INVESTIGADORES
CASAIS marilina
congresos y reuniones científicas
Título:
Progesterone reduced expression of sensory innervation markers and BDNF in endometriotic lesions in mice
Autor/es:
PAOLETTI GEUNA G; DELSOUC MB; SERÁN C; CONFORTI RA; VALLCANERAS SS; CASAIS M
Lugar:
San Luis
Reunión:
Congreso; XXXVII Reunión Anual de la Sociedad de Biología de Cuyo; 2019
Resumen:
Endometriosis (EDT) is a chronic gynecological disease that affects 10-15% of all women of reproductive age. The most common clinical signs of this pathology are menstrual irregularities, chronic pelvic pain, dysmenorrhea, dyspareunia, dysuria, fatigue, and depression. Progestin use can effectively treat the symptoms of endometriosis, although the mechanisms of action are not fully known. Therefore, the aim of this work was to elucidate whether Progesterone modifies the expression of sympathetic and sensory innervation markers, as well as neurotrophins, in endometriotic lesions induced in mice. EDT was induced experimentally by autotransplanting three pieces of the right uterine horn to the intestinal mesentery in C57BL/6 mice (Protocol CICUA-UNSL #B-262/17). From day 1 after the operation, the animals of the control group (n= 7) received sterile corn oil, while the animals of the experimental group (n= 7) received 2.5 mg Progesterone/kg bw/day (P0130 Sigma-Aldrich) subcutaneously for 1 month. The mice were then sacrificed by cervical dislocation and the lesions were removed and stored at -80°C for subsequent analysis of gene expression, by reverse transcription-polymerase chain reaction (RT-PCR), of tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), calcitonin gene-related peptide (CGRP), Substance P (SP), brain-derived neurotrophic factor (BDNF) and neural growth factor (NGF). The protein expressions of both neurotrophins BDNF and NGF were analyzed by an indirect enzyme‐linked immunosorbent assay (ELISA). Data were evaluated using T-test (p