INVESTIGADORES
PRADO carolina Del Valle
artículos
Título:
Detoxification of Cr(VI) in Salvinia minima is related to seasonal-induced changes of thiols, phenolics and antioxidative enzymes
Autor/es:
CAROLINA PRADO, EDUARDO PAGANO, FERNANDO PRADO, MARIANA ROSA
Revista:
JOURNAL OF HAZARDOUS MATERIALS.
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2012 p. 355 - 361
ISSN:
0304-3894
Resumen:
In this study, protein- and non-protein-thiol-containing compounds (THCC), soluble phenolics (SP), pro-line (Pro), proteins and malondialdehyde (MDA) contents, and antioxidative enzyme activities wereanalyzed in floating and submerged leaves of Salvinia minima to establish their role against Cr-inducedoxidative stress. We analyzed relationships among biochemical responses to different Cr(VI) concentra-tions to explore underlying mechanisms of Cr detoxification in plants growing under field conditionsduring summer and winter seasons. Significant increases in THCC were observed in submerged leavesfrom both seasons, while in floating leaves THCC increased only in summer being decreased in winter.Contrarily SP increased in floating leaves and decreased in submerged ones. MDA increased significantlyin winter-leaves, but in summer-leaves remained unchanged. Antioxidative enzymes, i.e. guaiacol peroxidase (G-POD), superoxide dismutase (SOD) and catalase (CAT) showed different activity patterns. G-PODsignificantly increased in Cr-treated leaves from both seasons, while SOD increased in submerged leavesonly, remaining practically unchanged in floating ones. CAT activity increased in floating leaves fromboth seasons, whereas in submerged ones was decreased or increased. Proteins increased in both leaftypes during summer whereas decreased or remained unchanged in winter. Pro increased in winter-submerged leaves only. Results show that seasonal-induced changes occur in all measured parameters