Circumscription and phylogeny of Apiaceae subfamily Saniculoideae based on chloroplast DNA sequences

CALVIÑO, C. I. AND DOWNIE, S. R.

MOLECULAR PHYLOGENETICS AND EVOLUTION

Academin Press Inc Elsevier Science

Lugar: San Diego, CA, USA; Año: 2007 vol. 44 p. 175 - 191

1055-7903

An estimate of phylogenetic relationships within Apiaceae subfamily Saniculoideae was inferred using data from the chloroplast DNAtrnQ-trnK 5-exon region to clarify the circumscription of the subfamily and to assess the monophyly of its constituent genera. Ninetyoneaccessions representing 14 genera and 82 species of Apiaceae were examined, including the genera Steganotaenia, Polemanniopsis, andLichtensteinia which have been traditionally treated in subfamily Apioideae but determined in recent studies to be more closely related toor included within subfamily Saniculoideae. The trnQ-trnK 5-exon region includes two intergenic spacers heretofore underutilized inmolecular systematic studies and the rps16 intron. Analyses of these loci permitted an assessment of the relative utility of these noncodingregions (including the use of indel characters) for phylogenetic study at diVerent hierarchical levels. The use of indels in phylogenetic analysesof both combined and partitioned data sets improves resolution of relationships, increases bootstrap support values, and decreaseslevels of overall homoplasy. Intergeneric relationships derived from maximum parsimony, Bayesian, and maximum likelihood analyses,as well as from maximum parsimony analysis of indel data alone, are fully resolved and consistent with one another and generally verywell supported. We conWrm the expansion of subfamily Saniculoideae to include Steganotaenia and Polemanniopsis (as the new tribe SteganotaenieaeC.I. Calviño and S.R. Downie) but not Lichtensteinia. Sister group to tribe Steganotaenieae is tribe Saniculeae, redeWned toinclude the genera Actinolema, Alepidea, Arctopus, Astrantia, Eryngium, Petagnaea, and Sanicula. With the synonymization of Hacquetiainto Sanicula, all genera are monophyletic. Eryngium is divided into “Old World” and “New World” subclades and within Astrantia sectionsAstrantia and Astrantiella are monophyletic.