INVESTIGADORES
CANOSA luis fabian
congresos y reuniones científicas
Título:
Ghrelin and nucb2/nesfatin-1: co-localization in brain and intestine, possible interaction with digestive enzymes in the intestine of pejerrey (Odontesthes bonariensis).
Autor/es:
BERTUCCI JI; BLANCO AM; SÁNCHEZ-BRETAÑO A; UNNIAPPAN S; CANOSA LF
Lugar:
Buenos Aires
Reunión:
Congreso; Reunion conjunta de sociedades de biociencia; 2017
Institución organizadora:
Sociedades de Biociencias de Argentina
Resumen:
Abstract: Ghrelin and nesfatin-1 are two metabolic peptideswith opposing actions on food intake regulation. Ghrelin is a potentorexigenic, mainly synthesized in the gastro-intestinal (GI) tract ofvertebrates, although is also present in central tissues. Nesfatin-1 is ananorexigenic peptide, mainly synthesized in the hypothalamus although it geneexpression was reported in the GI tract. The aim of this work was tocharacterize by immunohistochemistry the localization of ghrelin and nesfatin-1in brain and intestine of pejerrey (Odontesthes bonariensis), aspecies with commercial importance in South America. Additionally, the co-localizationof both peptides with enzymes involved in the digestion process such aslipoprotein lipase, aminopeptidase A, trypsin and sucrase-isomaltase in theintestine of pejerrey was studied. Results show that both ghrelin andnesfatin-1 have a similar anatomical distribution in brain, with the highestimmunoreactivity detected in some hypothalamic nuclei (such as the preopticnucleus and the posterior recess nucleus) and in the pineal gland, and lower levelsobserved in telencephalon, optic tectum, cerebellum and some hindbrain nuclei.Both peptides are presents and co-localize in some cells from the mucosa andsubmucosa layers of pejerrey intestine. Co-localization of ghrelin andnesfatin-1 with lipoprotein lipase, aminopeptidase A, trypsin andsucrase-isomaltase was found in intestine, in cells from the brush border andfrom the deep part of the mucosa and in the submucosa. In conclusion, in thiswork we present evidence for the presence and co-localization of ghrelin andnesfatin-1 in brain areas and intestine of pejerrey, which supports the role ofboth peptides in the food intake regulation. Additionally, the co-localizationof ghrelin or nesfatin-1 with digestive enzymes suggests a role for both peptideson nutrient assimilation in pejerrey.