INVESTIGADORES
CANOSA luis fabian
congresos y reuniones científicas
Título:
Sex Steroid Hormones Modulate NUCB2/Nesfatin-1 and Ghrelin in Goldfish
Autor/es:
BERTUCCI JI; BLANCO AM; UNNIAPPAN S; CANOSA LF
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Resumen:
Ghrelin (orexigenic) and nesfatin-1 (anorexigenic, encoded by nucleobindin-2/NUCB2 gene) play an important role in food intake and energy balance. The influence of sex steroid hormones in food intake has been demonstrated in fish. We studied the effects of estradiol (E2) and testosterone (T) on the expression of ghrelin, its receptor (GHSR), ghrelin-O-acyltransferase (GOAT), NUCB2 and its related peptide NUCB1 in goldfish. We implanted fish with pellets containing 100 µg/g BW of E2, T or without hormone (control). After 2.5 days, gene expression in gut (G), forebrain (Fb), hindbrain (Hb), hypothalamus (Hp) and pituitary (P) was measured. For both treatments we observed low ghrelin expression in Hp, while GOAT in G and GHSR in P showed an upregulation. NUCB2 expression was increased in Fb for T group and reduced in G and P under both treatments. NUCB1 showed overexpression in Fb and Hb for E2 treatment and in G, Fb and Hp for T treatment. These results show an overall anorexigenic effect of E2 and T, suggesting an interaction between these hormones and genes implicated in food intake regulation that may be part of the molecular basis for the seasonal changes observed in goldfish.