INVESTIGADORES
CANOSA luis fabian
congresos y reuniones científicas
Título:
Characterization of PACAP in two Latin American silverside fish (Atherinopsidae
Autor/es:
CHAVEZ-MALDONADO M; CÁRDENAS-REYGADAS R; KRAEMER MN; CANOSA LF
Lugar:
Juriquilla, Santiago de Queretaro
Reunión:
Encuentro; Second meeting of the NASCE; 2013
Institución organizadora:
North American Society for Comparative Endocrinology
Resumen:
Silverside fish in both Mexico and Argentina represent an important economical resources but their aquaculture fails to reach industrial levels and economical sustainability. One of the factors for this phenomenon is found in the slow growth rate of this fish. Therefore, it is important to study the endocrine regulation of growth in this species. In the present work pituitary adenyl cyclase activating polypeptide (PACAP) was cloned and the brain distribution was investigated in two members of Atherinopsidae family, Chirostoma humboldtianum and Odontesthes bonariensis. The isolated cDNAs encode for a signal peptide (1-25), a cryptic peptide (26-83) a PACAP related peptide (PRP; 84-128) and PACAP itself (131-168). In both species, two cDNA variants were found representing an alternative splicing of the same gene as it was found for other teleost. In the short form, exon 4 is spliced out and the transcript expresses PACAP but not PRP. Phylogenetic analysis shows that the transcripts from these species fall into the catfish-like clade. Distribution of PACAP expressing-cells in the brain was studied by immunohistochemistry (IHC) in C. humboldtianum and in situ hybridization (ISH) in O. bonariensis. PACAP mRNA was detected by ISH wide spread in the brain of O. bonariensis. PACAP expressing-cells were found in the telencephalon, the preoptic area, the ventral hypothalamus, thalamus and the hind brain. Similarly, IHC reveals PACAP expressing-cells in the brain of C. humboldtianum in the preoptic area and several hindbrain nuclei but not in the telencephalon or ventral hypothalamus. IHC also reveals axons across the telencephalon, the diencephalon, the optic tectum, cranial nerves and the pituitary. These results suggest that PACAP functions as a neuroendocrine peptide at the pituitary level as well as a neuromodulator in several brain areas. This work was partially supported by ANPCyT-Argentina (PICT 2006-074, PICT 2010-1493) and CONICET-Argentina (PIP 2011-0271) to LFC.