INVESTIGADORES
CANOSA luis fabian
congresos y reuniones científicas
Título:
Regulation of somatic growth and gene expression of the GH/IGF system and muscle growth biomarkers myostatin and myogenin by dietary lipids in juveniles of the pejerrey (Odontesthes bonariensis)
Autor/es:
GÓMEZ-REQUENI P; VILLIAN M; BOERI R; CANOSA LF
Lugar:
Ciudad Autonoma de Buenos Aires
Reunión:
Simposio; 7th Intrenational Symposium on Fish Endocrinology; 2012
Resumen:
Growth and mRNA levels of growth hormone receptors (GHRs), insulin-like growth factors (IGFs) and muscle growth biomarkers myostatin (MSTN) and myogenin (MyoG) were analyzed in juveniles of pejerrey fed with graded levels of dietary lipids (L) ranging 6%, 10% and 25%. At the end of the 14-week trial, growth rates were higher with L10 and L25 paralleled by a decrease in liver IGF-I expression. Hepatic GHR-I mRNA levels followed the same trend whereas GHR-II and IGF-II did not change. Muscle IGF-I expression slightly increased in groups L10 and L25 whereas GHR-I decreased in L10. GHR-II, IGF-II and MyoG mRNA levels increased in L6 while MSTN did not differ between treatments. These results suggest an apparent inhibition of hypertrophic mechanisms to counteract excessive muscle growth hyperplasia in groups L10 and L25. In the adipose tissue, a decrease of IGF-I mRNA levels was evident in group L10, whereas GHR-I did not change. Similarly, IGF-II and GHR-II mRNA levels were not modified although a slight and progressive increase was observed with the increase of the dietary lipids. Taking these results together, it is clear a particularly fine tuning of the GH/IGF system with 10% dietary lipid levels, suggesting that the energy demands for growing pejerrey were met in this condition. Grants: PICT10-1493, PIP0271.