INVESTIGADORES
CANOSA luis fabian
artículos
Título:
Effects of cholecystokinin and bombesin on pre-pro-somatostatin gene expression in goldfish forebrain
Autor/es:
CANOSA LF; PETER RE
Revista:
REGULATORY PEPTIDES
Referencias:
Año: 2004 vol. 121 p. 99 - 105
ISSN:
0167-0115
Resumen:
It was previously demonstrated that both cholecystokinin (CCK) and bombesin (BBS) stimulate growth hormone (GH) secretion ingoldfish. Both peptides induce satiety and it was speculated that they integrate satiation and the postprandial increase in GH circulatinglevels. In the present paper we investigated the effects of CCK and BBS on the forebrain expression of the somatostatin gene family ingoldfish to analyze if somatostatin peptides may be part of the effector mechanisms of CCK and BBS. We found that peripherally as well ascentrally administered CCK decreases mRNA levels of preprosomatostatin (PSS)-I that encodes for SRIF-14, having no effects on PSS-II andPSS-III, which encode for gSRIF-28 and [Pro2] SRIF-14, respectively. In addition, a direct action on the pituitary to stimulate GH release,this inhibition of PSS-I expression provides a possible mechanism for CCK to increase postprandial GH levels. On the other hand, BBSinhibits the forebrain expression of PSS-I and PSS-II but does not affect PSS-III regardless of the route of administration. We conclude thatthis could be the most likely mechanism of action of BBS to increase GH secretion, since there are few BBS-immunoreactive (IR) fibers andBBS binding sites in the anterior pituitary of goldfish.